猪A型塞内卡病毒分离鉴定及其VP1基因变异分析

doi:10.11843/j.issn.0366-6964.2019.09.025

Abstract

Abstract: In July, 2017, an outbreak of swine vesicular disease caused by unknown pathogen in a pig farm in southern China. In order to confirm the pathogen of the vesicular disease and understand its genetic evolution, the pathogens associated with vesicular disease detected by RT-PCR and virus isolation, electron microscopy test and the phylogenetic analysis of VP1 gene were performed. RT-PCR results indicated that Senecavirus A (SVA), responsible for the vesicular disease occurred in the farm in southern China, was detected. SVA were successfully isolated in BHK21 cells and named CH/FuJ1/2017 strain. The SVA isolates had the lowest homology with the prototype SVV-001 strain and the highest homology with USA-GBI29-2015 strain from the United States. The analysis based on the amino acid sequences of SVA VP1 gene showed that isolates of SVA were located in a relatively independent branch and compared with SVV-001 strain, there were six amino acids mutations in VP1 in 59, 62, 63, 93, 97 and 172, respectively. These amino acids mutations mainly located on BC loop and CD loop Ⅱ on VP1. In conclusion, this study showed that SVA isolates in China were genetic diversity, and there was close correlation among some SVA isolates from China and foreign SVA isolates.

CLC Number:

S852.659.6

LI Xiubo, LIU Cun, CUI Yudong, LIANG Lin, ZHANG Jianwei, CUI Shangjin. Isolation and Identification of Porcine Senecavirus A and Mutation Analysis on Its VP1 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1945-1950.

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Isolation and Identification of Porcine Senecavirus A and Mutation Analysis on Its VP1 Gene

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